Premature Expression of Foxp3 in Double-Negative Thymocytes

Premature Expression of Foxp3 in Double-Negative Thymocytes

Peripheral immune regulation depends on the generation of thymic-derived regulatory T (tTreg) cells to maintain self-tolerance and to counterbalance overshooting immune responses. The expression of the Treg lineage defining transcription factor Foxp3 in developing tTreg cells depends on TCR signaling during the thymic selection process of these T cells. In this study, we surprisingly identify F...

Surface expression of Notch1 on thymocytes: correlation with the double-negative to double-positive transition.

Notch1 plays a critical role in regulating T lineage commitment during the differentiation of lymphoid precursors. The physiological relevance of Notch1 signaling during subsequent stages of T cell differentiation has been more controversial. This is due in part to conflicting data from studies examining the overexpression or targeted deletion of Notch1 and to difficulties in distinguishing bet...

CD8 Single-Positive Thymocytes Thymocytes and Enhances the Production of the Maturation of Double-Negative Transgenic Expression of RasGRP1 Induces

Direct Detection of FoxP3 Expression in Thymic Double-Negative CD4−CD8− Cells by Flow Cytometry

Foxp3 expression is a marker of regulatory T cells (Treg), but how early it is expressed in the thymus is still not fully defined. In this study, we examined Foxp3 expression in double-negative (DN) CD4(-)CD8(-) T cell precursors in the thymus by flow cytometry. By increasing the number of collected cells from the conventional 10(4) cells up to more than 10(6) cells during flow cytometry, we fo...

Premature escape of double-positive thymocytes to the periphery of young mice. Possible role in autoimmunity.

Thymectomy of 3-day-old mice induces organ-specific autoimmune disease. To define a relationship between the development of T cells early in the neonatal period and autoimmunity, we studied the thymus and peripheral lymphoid tissues of 3-day-old mice. Lymph nodes, but not spleens, of 3- to 4-day-old mice contained a significant number of thymus-derived CD4+CD8+ cells that are phenotypically sim...